Total Body Research Articles

Clinical Implementation of Total-Body PET in China.

Total-body (TB) PET/CT is a groundbreaking tool that has brought about a revolution in both clinical application and scientific research. The transformative impact of TB PET/CT in the realms of clinical practice and scientific exploration has been steadily unfolding since its introduction in 2018, with implications for its implementation within the health care landscape of China. TB PET/CT's exceptional sensitivity enables the acquisition of high-quality images in significantly reduced time frames. Clinical applications have underscored its effectiveness across various scenarios, emphasizing the capacity to personalize dosage, scan duration, and image quality to optimize patient outcomes. TB PET/CT's ability to perform dynamic scans with high temporal and spatial resolution and to perform parametric imaging facilitates the exploration of radiotracer biodistribution and kinetic parameters throughout the body. The comprehensive TB coverage offers opportunities to study interconnections among organs, enhancing our understanding of human physiology and pathology. These insights have the potential to benefit applications requiring holistic TB assessments. The standard topics outlined in The Journal of Nuclear Medicine were used to categorized the reviewed articles into 3 sections: current clinical applications, scan protocol design, and advanced topics. This article delves into the bottleneck that impedes the full use of TB PET in China, accompanied by suggested solutions.

Dose Reduction in Pediatric Oncology Patients with Delayed Total-Body [18F]FDG PET/CT.

Our aim was to define a lower limit of reduced injected activity in delayed [18F]FDG total-body (TB) PET/CT in pediatric oncology patients. Methods: In this single-center prospective study, children were scanned for 20 min with TB PET/CT, 120 min after intravenous administration of a 4.07 ± 0.49 MBq/kg dose of [18F]FDG. Five randomly subsampled low-count reconstructions were generated using ¼, ⅛, [Formula: see text], and [Formula: see text] of the counts in the full-dose list-mode reference standard acquisition (20 min), to simulate dose reduction. For the 2 lowest-count reconstructions, smoothing was applied. Background uptake was measured with volumes of interest placed on the ascending aorta, right liver lobe, and third lumbar vertebra body (L3). Tumor lesions were segmented using a 40% isocontour volume-of-interest approach. Signal-to-noise ratio, tumor-to-background ratio, and contrast-to-noise ratio were calculated. Three physicians identified malignant lesions independently and assessed the image quality using a 5-point Likert scale. Results: In total, 113 malignant lesions were identified in 18 patients, who met the inclusion criteria. Of these lesions, 87.6% were quantifiable. Liver SUVmean did not change significantly, whereas a lower signal-to-noise ratio was observed in all low-count reconstructions compared with the reference standard (P < 0.0001) because of higher noise rates. Tumor uptake (SUVmax), tumor-to-background ratio, and total lesion count were significantly lower in the reconstructions with [Formula: see text] and [Formula: see text] of the counts of the reference standard (P < 0.001). Contrast-to-noise ratio and clinical image quality were significantly lower in all low-count reconstructions than with the reference standard. Conclusion: Dose reduction for delayed [18F]FDG TB PET/CT imaging in children is possible without loss of image quality or lesion conspicuity. However, our results indicate that to maintain comparable tumor uptake and lesion conspicuity, PET centers should not reduce the injected [18F]FDG activity below 0.5 MBq/kg when using TB PET/CT in pediatric imaging at 120 min after injection.

Investigating effect of standardized total body skin examination using sequence-networks

This study employs sequence-network analysis to investigate the influence of instructing a standardized procedure on total-body skin examination (TBSE) performance. A between-subjects study was conducted with thirty-one participants comprising medical students and attending physicians. Among these participants, fifteen were randomly assigned to the uninstructed group and sixteen to the instructed group. The participants' gaze and field of view were recorded using eye tracking glasses while performing TBSE on a male and a female standardized patient. The recordings were then transcribed to depict the examination process. The instructed group missed significantly fewer body parts (p = 0.045) and had higher time efficiency (p = 0.007) while examining the female patient, but no significant difference was observed for the male patient examination. Furthermore, the examination sequences of the instructed group contained lesser variability than the uninstructed group. Hence, implementing a standard optimal procedure to perform TBSE could minimize the likelihood of missing body parts, increase examination efficiency, and improve performance consistency. This study demonstrated the potential of sequence-network analysis to study human performance in sequential tasks.

Seasonal Changes in Body Composition in Elite Male Handball Players

Having knowledge of an athlete's body composition values enables individuals to assess their physical condition and performance. It has become increasingly important in recent years to demonstrate the significant relationship between body composition and athletes' physical performance. The aim of this study is to examine the changes in body composition of elite male handball players during preparation and competition periods (42 weeks). Twenty-three elite male handball players volunteered for this study The Tanita brand body analysis device was used to evaluate body composition and eight measurements were taken. Athletes were divided according to their playing positions (playmakers n=8 and goalkeepers n=4, wings n=7 and pivots n=4). 7 measurements were evaluated, which were weight (W), body fat percentage (BF%), fat mass (FM), muscle mass (MM), total body water (TBW), body mass index (BMI), and basal metabolic rate (BMR). Wing players had lower body fat percentage and body fat mass than all other playing positions and regardless of position, all athletes' body fat percentage and body fat mass significantly decreased at T2 (p&amp;lt;0.05). As a result, increase in body fat percentage and fat mass was observed during the competition period, and this increase continued (p&amp;lt;0.05). The increase in fat mass during the competition period may be due to different training content and higher training intensity during the preparation period, changes in nutrition habits due to camps, long trips, and tournaments, as well as the ef-fects of fatigue and stress. Further studies are needed in these areas.

Assessment of Body Mass Index, Body Composition, Physical Activity, and Dietary Preferences in University Students: A Pilot Study

(1) Background: Body composition analysis, particularly the assessment of the amount and distribution of body fat and muscle mass in young people, is of considerable clinical importance for the detection of nutritional disorders. (2) Methods: University students aged 19–25 years had their body composition measured using a bioimpedance (BIA) device InBody 370S. Furthermore, a questionnaire survey was performed using the questionnaires: factors that influence your choice of food (FCQ); food preference questionnaire for adolescents and adults (FPQ); the international physical activity questionnaire (IPAQ). (3) Results: Body mass index (BMI) values were within a normal range in 89.5% of men and 77.9% of women, while statistically significant differences between the sexes were confirmed for all body parameters (p &lt; 0.001; p = 0.025). The waist-to-hip ratio (WHR) and percentage body fat (PBF) were abnormally high in 50.4% and 44.3% of women, respectively. High values of skeletal muscle mass (SMM), protein, minerals, and bone mineral content (BMC) were identified in 36.8% of men. A total of 88 students (66.7% of men and women) had a higher level of physical activity, i.e., achieved metabolic equivalent (MET) values of more than 3000 per week. (4) Conclusions: BMI does not always have explanatory power for assessing body weight, as it does not consider the percentage distribution of fat and non-fat body mass in the total body weight. Physical activity and a varied diet have a positive effect on achieving optimal body weight and are effective in preventing nutritional disorders (such as obesity and malnutrition) and associated health problems.

Delineating Gastrointestinal Dysfunction Variants in Severe Burn Injury Cases: A Retrospective Case Series with Literature Review

Background: Severe burns can significantly impact various organ systems, including the gastrointestinal (GI) system. GI complications are frequently observed in patients with over 20% total body surface area (TBSA) burn. Objectives: This case series delves into the intricate phenomenology of post-burn GI dysfunction, challenging conventional cause-and-effect paradigms. Our aim is to discern, comprehend, and explore variables influencing positive and negative outcomes, laying the foundation for further research given the current heterogeneity in the literature. Methods: Severe burn patients with GI dysfunction identified between April 1, 2022, and July 31, 2022, from the institutional database are included in this retrospective case-series, and comparisons were made across baseline and treatment conditions across participants. Data were collected on demographics, burn characteristics, complications, and treatment outcomes. Results: We analysed 12 patients with severe burns and GI dysfunction and categorized them into two patterns: Pattern A, characterised by early onset symptoms, gastric and small bowel dilatation, and a relatively benign course with high recovery rates was observed in 6 patients; and Pattern B, characterised by late-onset symptoms, colonic dilatation, shock, and a high mortality rate due to megacolon was seen in 6 patients. Conclusion: The post-burn GI dysfunction observed in our study is a complex interplay of multiple factors. Adequate fluid resuscitation, timely excision of necrotic tissue, staged food ingestion, specific nutrient administration, and appropriate use of antibiotics and judicious use of selective digestive decontamination (SDD) are essential strategies to prevent and treat this syndrome.

Performance of an automated total body mapping algorithm to detect melanocytic lesions of clinical relevance

ImportanceTotal body photography for skin cancer screening is a well-established tool allowing documentation and follow-up of the entire skin surface. Artificial intelligence-based systems are increasingly applied for automated lesion detection and diagnosis. Design and patientsIn this prospective observational international multicentre study experienced dermatologists performed skin cancer screenings and identified clinically relevant melanocytic lesions (CRML, requiring biopsy or observation). Additionally, patients received 2D automated total body mapping (ATBM) with automated lesion detection (ATBM master, Fotofinder Systems GmbH). Primary endpoint was the percentage of CRML detected by the bodyscan software. Secondary endpoints included the percentage of correctly identified “new” and “changed” lesions during follow-up examinations. ResultsAt baseline, dermatologists identified 1075 CRML in 236 patients and 999 CRML (92.9%) were also detected by the automated software. During follow-up examinations dermatologists identified 334 CRMLs in 55 patients, with 323 (96.7%) also being detected by ATBM with automated lesions detection. Moreover, all new (n = 13) or changed CRML (n = 24) during follow-up were detected by the software. Average time requirements per baseline examination was 14.1 min (95% CI [12.8–15.5]). Subgroup analysis of undetected lesions revealed either technical (e.g. covering by clothing, hair) or lesion-specific reasons (e.g. hypopigmentation, palmoplantar sites). ConclusionsATBM with lesion detection software correctly detected the vast majority of CRML and new or changed CRML during follow-up examinations in a favourable amount of time. Our prospective international study underlines that automated lesion detection in TBP images is feasible, which is of relevance for developing AI-based skin cancer screenings.

How stroke-related dysphagia relates to quality of life: the mediating role of nutritional status and psychological disorders, and the moderating effect of enteral nutrition mode.

Although stroke-related dysphagia has been shown to influence quality of life (QOL), the underlying mechanisms have yet to be uncovered. This study aims to investigate the mediating role of nutritional status and psychological disorders in the relationship between stroke-related dysphagia and QOL in stroke patients and explore the moderating effect of enteral nutrition mode. In 2022, A questionnaire survey using stratified random sampling was conducted on 5,322 stroke patients with dysphagia, including Functional Oral Intake Scale (FOIS), Swallowing Quality of Life Questionnaire, Patient Health Questionnaire-9 (PHQ-9), and Generalized Anxiety Disorder-7 (GAD-7) to assess dysphagia, QOL and psychological disorders, respectively, for each participant. Records of serum albumin, Hemoglobin, Total serum protein, serum prealbumin and Body mass index were enrolled to assess nutritional status. FOIS demonstrated a significant positive predictive effect on QOL. Nutritional status and psychological disorders (PHQ-9 and GAD-7) mediated the relationship between FOIS and QOL. Nutritional status-psychological disorders showed a chain mediation effect in the relationship between FOIS and QOL. The moderating effect of enteral nutrition mode was observed. The mediating role of nutritional status and psychological disorders with moderating effect of enteral nutrition mode in the relationship between dysphagia and QOL in stroke patients was found.

Maternal separation influences hepatic drug-metabolizing CYP450 gene expression without pathological changes in adult mice.

The principal motive of this study is to explore the influence maternal separation (MS) exhibits on the mRNA expression of major drug metabolizing-cyp450s in parallel with the assessment of pathological changes that can be induced by MS in the livers of experimental mice. Eighteen Balb/c mouse pups, comprising of both males and females, were separated from their mothers after birth. Following a six-week period during when the pups became adults, the mice were sacrificed and their livers were isolated for analysis of weight, pathohistological alterations, and the mRNA expression of drug metabolizing cyp450 genes: cyp1a1, cyp3a11, cyp2d9, and cyp2c29. The study demonstrated that MS markedly downregulated (p<0.05) the mRNA expression of all tested drug-metabolizing cyp450s in livers of female and male mice. Furthermore, the mRNA levels of major drug-metabolizing cyp450s were notably lower (p<0.05) in livers of female MS mice as compared with male MS mice. It was found that values of the total body weight and liver weight of MS mice did not vary significantly (p>0.05) from those of the control groups. Additionally, histological examination revealed that the hepatic tissue of MS mice was normal, similar to that of the control mice. In summary, MS downregulates the gene expression of major hepatic drug-metabolizing cyp450s without inducing pathological alterations in the livers of mice. These findings provide an explanation for the heterogeneity in pharmacokinetics and drug response of patients with early life stress.

Feather meal processing methods impact the production parameters, blood biochemical indices, gut function, and hepatic enzyme activity in broilers.

This study investigated the effects of feather meal (FM) processing methods on production parameters, blood biochemical indices, intestinal morphology, digestive and hepatic enzyme activities, and gastrointestinal tract pH and microflora of broilers. A total of 480-day-old male broilers were used for 42 days in a completely randomized design with eight treatments and five replicates (12 chicks/replicate). Treatments were: (1) A control diet (without FM), (2) Diet containing 4% raw FM (RFM), (3) Diet containing 4% processed feather meal (PFM) by autoclave (Au-PFM), (4) Diet containing 4% fermented FM (FFM) by Bacillus licheniformis (Bl-FFM), (5) Diet containing 4% FFM by Bacillus subtilis (Bs-FFM), (6) Diet containing 4% FFM by Aspergillus niger (An-FFM), (7) Diet containing 4% FFM by B. licheniformis+B. subtilis+A. niger (Co-FFM), and (8) Diet containing 4% PFM by an enzyme (En-PFM). Results showed that in the FFMs the contents of ash, ether extract, total volatile nitrogen, and amino acids including Lys, Met, Thr, Trp, His, Leu, Gly, Ile, Phe, and Tyr increased (P < 0.05), while crude fiber, crude protein, and dry matter content decreased (P < 0.05). Compared with the control, the Co-FFM diet had no significant differences (P > 0.05) in total body weight gain (2,827 vs. 2,791g/chick), total feed intake (5,018 vs. 4,991g/chick), European production efficiency factor (375 vs. 377), European broiler index (371 vs. 371), and feed conversion ratio (1.77 vs. 1.78g/g). Feeding FFM decreased (P < 0.05) serum total cholesterol (1.46-fold), triglyceride (1.61-fold), VLDL-C (1.61-fold), and LDL-C (2.27-fold) compared to the control. Also, FFM increased (P < 0.05) villus height (1,045 to 1,351, 661 to 854, and 523 to 620 μm), and villus height to crypt depth ratio (6.15 to 8.45, 4.55 to 7.04, and 4.27 to 5.45), in the duodenum, jejunum, and ileum respectively compared to the control. Compared to the control, the Co-FFM diet increased (P < 0.05) protease (34, 39, and 45 %) in the pancreas, duodenum, and jejunum, as well as amylase (73, and 97 %) activities in the duodenum, and jejunum respectively. Diets containing FFM reduced (P < 0.05) pH in the crop, gizzard, and ileum, and decreased (P < 0.05) Escherichia coli (6.12 to 5.70) count in ileum compared with the control. The Co-FFM diet increased (P < 0.05) lactic acid bacteria count in crop (6.77 to 7.50) and ileum (6.94 to 7.73), also decreased (P < 0.05) coliforms (6.31 to 5.75) count in ileum compared to the control. In conclusion, FM fermentation, particularly Co-FFM, improves the nutritional value of FM, converting it into a decent source of dietary protein for broilers.

The association between previous use of anti-obesity medication and semaglutide weight loss outcomes.

To compare weight loss outcomes between patients starting semaglutide who had previously been on another anti-obesity medication (AOM) compared to those who were AOM-naïve. We performed a retrospective study in patients with overweight or obesity taking semaglutide for weight loss for a duration of 3 to 12 months. Our primary endpoint was assessment of percentage of total body weight loss (TBWL) in patients who started semaglutide as their first AOM (AOM-naïve) compared to those who started semaglutide and had previously taken another AOM (non-AOM-naïve). The secondary outcome was a comparison of the proportions of patients achieving ≥5%, ≥10%, ≥15% and ≥20% TBWL between the groups. Our endpoints were analysed using independent t-tests and ANOVA/ANCOVA for continuous variables and Pearson's test for categorical variables. This study included 305 patients. Outcomes of semaglutide treatment were superior in AOM-naïve patients (n = 231) compared to non-AOM-naïve patients (n = 74) at 3 (6.3% vs. 3.8%), 6 (10.6% vs. 6.7%), 9 (14.0% vs. 9.1%) and 12 months (14.3% vs. 10.6%; p < 0.0001 at 3, 6 and 9 months, and p = 0.01 at 12 months). A greater proportion of patients in the AOM-naïve group achieved a TBWL ≥ 15% (48% vs 21%; p = 0.02) and ≥20% (27% vs 4% p < 0.01) at 12 months. The use of semaglutide in patients with previous intake of other AOMs was associated with inferior weight loss outcomes in comparison to patients who were AOM-naïve.

An evaluation of ankle and foot bolus in paediatric modulated arc total body irradiation (MATBI).

This retrospective planning study aimed to evaluate the role of bolus in achieving dose uniformity in the ankles and feet in paediatric patients undergoing Modulated Arc Total Body Irradiation (MATBI) treatment and to identify patient factors that may negate or warrant its use. The clinically treated plans of 20 paediatric patients who received MATBI treatment utilising ankle and foot bolus (Bolus plan) were compared with two retrospectively generated plans; a plan with bolus removed and no re-optimisation (No Bolus plan), and a re-optimised plan without bolus attempting to achieve equal dosimetry to the clinical plan via monitor unit adjustment (MU plan). Descriptive statistics were used to evaluate the dose uniformity criteria of ±10% coverage of the reference dose (RD) for each subregion of the ankle and foot for the three plans. The impact of patient height, weight, and age at the time of treatment was evaluated using Spearman's correlation. Variation in doses >10% RD was minimal across the three plans, with an average D1cc difference < 0.4Gy. For the ankle and foot regions in the Bolus plans, the volume receiving at least 90% of the RD (V90) was on average > 92%. In No Bolus and MU plans, there was an average reduction of 24.5% and 23.2% V90 coverage respectively in the toes. Spearman's correlation suggests height has the strongest relationship to D1cc. This study validated the continued use of ankle and foot bolus to achieve dosimetric goals for paediatric MATBI treatments, particularly V90 coverage across all heights.

Water Nutriture: Essential but Misunderstood

Abstract: In their search for life, space scientists are looking for traces of water on other planets in the solar system because of the well accepted knowledge that water is important for all living systems. Water makes up 60-75% of human body weight. A loss of just 4% of total body water can lead to severe dehydration, and a loss of 15% is fatal. What makes water so essential for survival? Though life on earth is described as a carbon-based phenomenon, it would not be amiss to also call it a water-based phenomenon, as water is the universal solvent that dissolves all biological molecules. Despite this deep understanding of both the chemistry and the biochemical importance of water in human physiology, questions still remain regarding what is the required consumption of water? What type of water is to be ingested? In the context of Nutrition, water is not only an essential nutrient but is also an important source of antinutrients and toxins. Another question that remains ambiguous is: How much water is essential? If it is a nutrient, does it have an RDA? Can one suffer and die from water intoxication? This review puts together in simple terms what one needs to know about water as an essential nutrient in our diet.

Dioscin alleviates aplastic anemia through regulatory T cells promotion

ABSTRACT Objectives: Aplastic anemia (AA) is one of the immune-mediated bone marrow failure disorders caused by multiple factors, including the inability of CD4 + CD25 + regulatory T cells (Tregs) to negatively regulate cytotoxic T lymphocytes (CTLs). Dioscin is a natural steroid saponin that has a similar structure to steroid hormones. The purpose of this study is to look into the effect of Dioscin on the functions of CD4 + CD25+ Tregs in the AA mouse model and explore its underlying mechanism. Methods: To begin with, bone marrow failure was induced through total body irradiation and allogeneic lymphocyte infusion using male Balb/c mice. After 14 consecutive days of Dioscin orally administrated, the AA mouse model was tested for complete blood counts, HE Staining of the femur, Foxp3, IL-10 and TGF-β. Then CD4 + CD25+ Tregs were isolated from splenic lymphocytes of the AA mouse model, Tregs and the biomarkers and cytokines of Tregs were measured after 24 h of Dioscin intervention treatment in vitro. Results: Dioscin promotes the expression of Foxp3, IL-10, IL-35 and TGF-β, indicating its Tregs-promoting properties. Mechanistically, the administration of Dioscin resulted in the alteration of CD152, CD357, Perforin and CD73 on the surface of Tregs, and restored the expression of Foxp3. Conclusion: Dioscin markedly attenuated bone marrow failure, and promoted Tregs differentiation, suggesting the maintenance of theimmune balance effect of Dioscin. Dioscin attenuates pancytopenia and bone marrow failure via its Tregs promotion properties.

Improved Short-Term Prognosis of Pediatric Partial-Thickness Burns: Emergency Conservative Debridement Under Topical Anesthesia.

Early wound management for pediatric patients with partial-thickness burns in the emergency department remains debatable. This study aims to evaluate the value of emergency conservative debridement under topical anesthesia in improving short-term prognosis of pediatric partial-thickness burns. This retrospective cohort study enrolled children with partial-thickness thermal burns presenting to the emergency department within 6 hours postburn. All the enrolled patients were divided into 2 groups: the debridement group and the dressing group. The associations between emergency conservative debridement and time to reepithelialization was analyzed by using Kaplan-Meier curves with log rank test and multivariate Cox regression analysis. Moreover, the associations between emergency conservative debridement and in-hospital cost and length of stay were also evaluated. All baseline characteristics between groups were comparable (all P > 0.05). Emergency conservative debridement under topical anesthesia significantly decreased the median value of time to reepithelialization (13 vs 14 days, P = 0.02). Cox regression analysis showed that emergency conservative debridement significantly improved wound reepithelialization after adjusting for burn size (odds ratio, 4.07; 95% confidence interval, 1.64-10.11; P < 0.01). The mean length of stay of patients receiving conservative wound debridement was lower than that of patients in the wound dressing group (14.3 ± 7.3 vs 18.8 ± 10.4 days, P < 0.01), but not in terms of mean in-hospital cost per 1% total body surface area (2.8 ± 1.9 vs 3.0 ± 2.1 × 103 RMB per 1% total body surface area, P = 0.58). Emergency conservative debridement of pediatric partial-thickness burns under topical anesthesia significantly improves the wound healing outcomes without increasing health care burden.

The Impact of Effective Mass on the Strength of Side and Turning Kick in Taekwon-Do Male Practitioners

Background: One of the elements of improving the striking power of combat sports athletes is the ability to use their body mass in an efficient manner; this can improve movement technique and thus increase combat effectiveness. Therefore, the aim of this study was to gain knowledge about the influence of the effective mass obtained on the strength of turning and side kicks in the context of lateralization. Material and methods: The study involved four adult taekwon-do ITF (International Taekwon-do Federation) competitors. Acceleration and force data were obtained by mounting a wireless IMU sensor manufactured by Noraxon, which was synchronized with a force plate. Results: The median force pressure values amounted to 2661.53 N for the turning kick and 4596.15 N for the side kick, with foot acceleration of 150.56 m/s2 and 74.34 m/s2, respectively. The calculated median effective mass for the turning kick was 20.12%, and for the side kick, it was 73.09% of the total body mass. The conducted analysis indicates a lack of statistically significant differences between the right and left leg in the obtained kinetic variable values (p &gt; 0.05). Conclusions: Our research suggests that the side kick achieves, on average, higher force values than the turning kick. The noted correlation between the three variables informs us that the greater the effective mass, the greater the force pressure and the smaller the foot acceleration, which is consistent with other studies. The lack of lateralization in the limbs performing the kicks is consistent with another study.

Effect of exercise-induced body fluid redistribution on body composition in males using dual energy X-ray absorptiometry

ABSTRACT We studied the effect of exercise-induced body fluid redistribution on dual-energy x-ray absorptiometry (DXA) body composition scores. Thirty males completed 30-min of upper-body exercise (UBE), lower-body exercise, and seated non-exercise control (NEC). ANOVA determined interactions between experimental conditions and measurements on body composition variables. For UBE, mean pre to post differences were found on tissue fat (M = 0.35 ± 0.12%; CI95%diff = 0.10 to 0.59%; p = 0.007), region fat (M = 0.32 ± 0.11%; CI95%diff = 0.09 to 0.55%; p = 0.008), lean mass (M = 0.27 ± 0.01 kg; CI95%diff = 0.18 to 0.37 kg; p ≤ 0.0001), and total mass (M = 0.27 ± 0.05 kg; CI95%diff = 0.17 to 0.36 kg; p ≤ 0.0001). Mean tissue pre to post differences were found for the total body in the NEC (M = 0.10 ± 0.04 kg; CI95%diff = 0.03 to 0.18 kg; p = 0.008), UBE (M = 0.19 ± 0.03 kg; CI95%diff = 0.14 to 0.24 kg; p ≤ 0.0001), and LBE (M = 0.31 ± 0.04 kg; CI95%diff = 0.24 to 0.39 kg; p ≤ 0.0001) conditions. High absolute reliability was found within experimental conditions. These findings have practical implications for technicians, since acute exercise elicited small changes in body composition scores using DXA.

Effect of dexmedetomidine on fluid resuscitation in burn-injured patients.

Fluid creep occurs when resuscitation after extensive burn injury reaches volumes higher than predicted. Since this has been described in patients with high opioid requirements, continuous analgesics and sedatives, including dexmedetomidine, have been avoided during resuscitation. This study sought to describe the impact of dexmedetomidine on fluid resuscitation requirements. This retrospective cohort study included adult patients with burns greater than 20% total body surface area (TBSA) resuscitated between January 2017 and June 2022 at a regional burn center. Patients deceased within 48 hours of burn were excluded. Primary outcome was volume of fluid required in the first 24 and 48 hours post-burn. Secondary outcomes were the incidence of fluid-related adverse events within 7 days post-burn. A total of 170 patients were included: 55 in the dexmedetomidine cohort and 115 in the control cohort. After propensity matching for variables associated with fluid creep, the dexmedetomidine cohort required 4.2 ± 1.7mL/kg/%TBSA in the first 24 hours compared to 3.6 ± 1.1mL/kg/%TBSA in the control cohort (p=0.03). The difference was no longer significant at 48 hours (p=0.11). There were no differences in the incidence of acute respiratory distress syndrome, delayed escharotomy/fasciotomy, intraabdominal hypertension, or renal replacement therapy. Dexmedetomidine exposure during acute resuscitation resulted in increased fluid requirements in the first 24 hours, suggesting it is independently associated with fluid creep; however, this increase was not sustained at 48 hours. Clinical significance of this finding is unclear as there was no increase in adverse events related to excessive fluid resuscitation between cohorts.

Multiple signs of ecosystem change in the zooplankton community of a large temperate lake

Large lakes, such as Lake Simcoe, Ontario, Canada, are undergoing significant change due to local and global stressors. Uni- and multivariate analyses of Lake Simcoe’s zooplankton community from 1986 to 2012 indicated multiple events of ecosystem change that were synchronous across three lake stations. In the mid-1990s, shifts in zooplankton species abundance and richness, and total cladoceran body size were strongly correlated with the invasion of the zooplanktivore, Bythotrephes cederstroemii. In the early 2000s, additional shifts in zooplankton abundance, as well as copepod body size, coincided with increased water clarity (linked to filter feeding by the invader Dreissena polymorpha) and hypolimnetic water temperature. Further community changes occurred in the 2000s when Bythotrephes declined and many vulnerable cladoceran species recovered. However, the Lake Simcoe community did not fully return to its pre-invasion state as the cold-water herbivores, Daphnia longiremis and Daphnia pulicaria, remained absent. The Lake Simcoe zooplankton community illustrates ongoing ecosystem change that propagated throughout the lake food web, and may be reflected in other lakes experiencing global stressors of climate change and species invasions.

The Effectiveness of Low-Carb Diet vs Low-Fat Diet on Body Composition in People with Obesity: A Literature Review

Background: Obesity is still become a serious problem today. Obesity is caused by excessive adipose tissue. One of many factors that contribute to a person's obesity is food intake. Excess carbohydrate and fat intake will be stored in the form of triglycerides in adipose tissue. In the meantime, Low-Carb Diet (LCD) and Low-Fat Diet (LFD) are one of the most popular treatments on obesity. However there are many pros and cons related to each diet based on several studies. Objectives: The indicated study aims to determine the effectiveness of LCD and LFD on body composition in people with obesity. Methods: The study was conducted through journal and literature review, based on five journal publications, filtered by related keywords. In accordance to inclusion and exclusion criteria within the last ten years in Pubmed/Medline database, Science Direct, and Wiley Online Library with the keywords "Low-Carb Diet", "Low-Fat Diet", "Body Mass Index", "Lipid Levels", "Adipose Tissue", "Obese", and "Body Water". Discussion: Total body mass and fat mass decreased significantly after being given LCD intervention compared to LFD. The group that was given two dietary interventions also losing weight, but there was no changes in body water. In addition, negative effects were found from the LCD and LFD interventions such as constipation, fatigue, polyuria, nausea, vomiting, changes in appetite, and headaches. Kidney failure, ketosis, and premature coronary artery also occurred in the group that was given with LCD intervention. Conclusions: LCD and LFD interventions can affect body composition of people with obesity.